Recognition of isozymes via lanthanide ion incorporated polymerized liposomes

Adekunle I. Elegbede; Manas K. Haldar; Sumathra Manokaran; Sanku Mallik; D. K. Srivastava

doi:10.1039/B709815D

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Recognition of isozymes via lanthanide ion incorporated polymerized liposomes†

Adekunle I. Elegbede,^a Manas K. Haldar,^a Sumathra Manokaran,^b Sanku Mallik*^a and D. K. Srivastava*^b

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* Corresponding authors

^a Department of Pharmaceutical Sciences, North Dakota State University, Fargo, North Dakota, USA
E-mail: sanku.mallik@ndsu.edu
Fax: 701-231-8333
Tel: 701-231-7888

^b Department of Chemistry, Biochemistry and Molecular Biology, North Dakota State University, Fargo, North Dakota, USA
E-mail: dk.srivastava@ndsu.edu
Fax: 701-231-8831
Tel: 701-231-7831

Abstract

We report the selective recognition of carbonic anhydrase isozymes based on the excited-state lifetimes of chelated Eu³⁺ ions incorporated in polymerized liposomes.

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Supplementary files

Synthesis of polymerizable inhibitor lipid 3. Preparation and polymerization of the liposomes. Estimation of Eu ³⁺ concentration on the outside surface of the liposomes. Cloning, expression and purification of carbonic anhydrase isozymes. Luminescence titration and determination of excited state lifetime for Eu ³⁺. Luminescence decay measurements. Determination of dissociation constants. Fig. S1: Time-resolved emission spectra of Eu ³⁺-incorporated polymerized liposomes as a function of increasing concentrations of CA I, CA II, CA XII and bovine erythrocyte CA PDF (432K)

Article information

DOI: https://doi.org/10.1039/B709815D
Article type: Communication
Submitted: 30 Jun 2007
Accepted: 17 Aug 2007
First published: 29 Aug 2007

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Chem. Commun., 2007, 4495-4497

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Recognition of isozymes via lanthanide ion incorporated polymerized liposomes

A. I. Elegbede, M. K. Haldar, S. Manokaran, S. Mallik and D. K. Srivastava, Chem. Commun., 2007, 4495 DOI: 10.1039/B709815D

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Chemical Communications

Recognition of isozymes via lanthanide ion incorporated polymerized liposomes†

Abstract

Supplementary files

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Recognition of isozymes via lanthanide ion incorporated polymerized liposomes

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