Reversible photopadlocking on double-stranded DNA

Kenzo Fujimoto; Shigeo Matsuda; Yoshinaga Yoshimura; Takehiro Ami; Isao Saito

doi:10.1039/B707524C

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Chemical Communications

Issue 28, 2007

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Reversible photopadlocking on double-stranded DNA

Kenzo Fujimoto , Shigeo Matsuda , Yoshinaga Yoshimura , Takehiro Ami and Isao Saito

Chem. Commun., 2007, 2968-2970

DOI: 10.1039/B707524C
Received 18 May 2007, Accepted 12 Jun 2007
First published on the web 22 Jun 2007

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We describe a highly efficient method for reversible photocircularization of oligonucleotide (ODN) on a double-stranded DNA template: 5-carboxyvinyl-2′-deoxyuridine-containing ODN was reversibly circularized around the target sequence of the double-stranded plasmid DNA resulting in formation of a catenated plasmid.

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Kenzo Fujimoto
Shigeo Matsuda
Yoshinaga Yoshimura
Takehiro Ami
Isao Saito

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Chemical Communications

Reversible photopadlocking on double-stranded DNA

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