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Issue 12, 2007
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Quantitative detection of protein expression in single cells using droplet microfluidics

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Abstract

We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.

Graphical abstract: Quantitative detection of protein expression in single cells using droplet microfluidics

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Publication details

The article was received on 19 Dec 2006, accepted on 16 Jan 2007 and first published on 26 Jan 2007


Article type: Communication
DOI: 10.1039/B618570C
Citation: Chem. Commun., 2007, 1218-1220
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    Quantitative detection of protein expression in single cells using droplet microfluidics

    A. Huebner, M. Srisa-Art, D. Holt, C. Abell, F. Hollfelder, A. J. deMello and J. B. Edel, Chem. Commun., 2007, 1218
    DOI: 10.1039/B618570C

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