A short-chain dehydrogenase/reductase from Vibrio vulnificus with both blue fluorescence and oxidoreductase activity

Karen Marie Polizzi; Desmond Antoine Moore; Andreas Sebastian Bommarius

doi:10.1039/B616763B

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Chemical Communications

Issue 18, 2007

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A short-chain dehydrogenase/reductase from Vibrio vulnificus with both blue fluorescence and oxidoreductase activity

Karen Marie Polizzi , Desmond Antoine Moore and Andreas Sebastian Bommarius

Chem. Commun., 2007, 1843-1845

DOI: 10.1039/B616763B
Received 16 Nov 2006, Accepted 23 Jan 2007
First published on the web 12 Feb 2007

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A dual function blue fluorescent protein from Vibrio vulnificus is also an NADPH-dependent oxidoreductase, rendering it a useful tool for biophysical studies.

Supplementary Info

Codon optimized DNA sequence of wild-type BFP, oligonucleotides used in synthesis, gene synthesis procedure, SDS-PAGE gel of purified proteins
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Chemical Communications

A short-chain dehydrogenase/reductase from Vibrio vulnificus with both blue fluorescence and oxidoreductase activity

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