Jump to main content
Jump to site search

Issue 7, 2007
Previous Article Next Article

Construction of di-scFv through a trivalent alkyneazide 1,3-dipolar cycloaddition

Author affiliations

Abstract

Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.

Graphical abstract: Construction of di-scFv through a trivalent alkyne–azide 1,3-dipolar cycloaddition

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Aug 2006, accepted on 30 Oct 2006 and first published on 28 Nov 2006


Article type: Communication
DOI: 10.1039/B611636A
Citation: Chem. Commun., 2007,0, 695-697
  •   Request permissions

    Construction of di-scFv through a trivalent alkyneazide 1,3-dipolar cycloaddition

    A. Natarajan, W. Du, C. Xiong, G. L. DeNardo, S. J. DeNardo and J. Gervay-Hague, Chem. Commun., 2007, 0, 695
    DOI: 10.1039/B611636A

Search articles by author

Spotlight

Advertisements