One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device†
Abstract
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.