Automated capture and on-column detection of biotinylated DNA on a disposable solid support

Michael Decuir; Ilkka Lähdesmäki; Andrea D. Carroll; Jaromir Ruzicka

doi:10.1039/B705617F

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Analyst

Issue 8, 2007

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Automated capture and on-column detection of biotinylated DNA on a disposable solid support

Michael Decuir , Ilkka Lähdesmäki , Andrea D. Carroll and Jaromir Ruzicka

Analyst, 2007, 132, 818-822

DOI: 10.1039/B705617F
Received 13 Apr 2007, Accepted 14 Jun 2007
First published on the web 02 Jul 2007

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Abstract
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This work comprises the development of a technique for the capture of single-stranded DNA on a solid support combined with in situ quantification. The capture is based on the strong and selective interaction between biotinylated DNA and streptavidin-coated agarose beads. Sequential Injection in the lab-on-valve format allows for automated manipulation of all components including the building and disposal of bead columns. Detection was accomplished using the OliGreen fluorescent dye and optimization of the assay achieved a limit of detection of 111 pg ssDNA, with a total assay time of roughly 2.5 min per sample.

Abstract from Analytical Abstracts 2008

This work comprises the development of a technique for the capture of single-stranded DNA on a solid support combined with in situ quantification. The capture is based on the strong and selective interaction between biotinylated DNA and streptavidin-coated agarose beads. Sequential injection in the lab-on-valve format allows for automated manipulation of all components including the building and disposal of bead columns. Detection was accomplished using the OliGreen fluorescent dye and optimization of the assay achieved a limit of detection of 111 pg ssDNA, with a total assay time of roughly 2.5 min per sample.

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