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Issue 9, 2007
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Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry

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Abstract

Urine samples obtained from drug abusers were screened for drugs of abuse and their metabolites using DESI-MS and the results obtained were compared to results obtained from GC–MS experiments. The detected analyte classes included amphetamines, opiates, cannabinoids and benzodiazepines. The compounds detected were codeine, morphine, oxymorphone, 11-nor-9-carboxy-Δ9-tetrahydrocannabinol, Δ9-tetrahydrocannabinol, alprazolam, temazepam, oxazepam, N-desmethyldiazepam (nordiazepam) and hydroxytemazepam. Identities of all the analytes were confirmed by tandem mass spectrometry, matching MS/MS spectra with authentic standard compounds. The concentrations of the analytes in the samples were obtained from semi-quantitative GC–MS studies and were in the range of 270–22 000 ng mL−1. The analytes could be detected by DESI even after a hundred-fold dilution indicating that the sensitivity of DESI was more than adequate for this study. Selectivity in the DESI-MS measurements for different kinds of analytes could be increased further by optimizing the spray solvent composition: the use of an entirely aqueous solvent enhanced the signal of polar analytes, such as the benzodiazepines, whereas the use of a spray solvent with a high organic content increased the signal of less polar analytes, such as codeine and morphine.

Graphical abstract: Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry

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Publication details

The article was received on 08 Mar 2007, accepted on 15 Jun 2007 and first published on 10 Jul 2007


Article type: Paper
DOI: 10.1039/B703524A
Citation: Analyst, 2007,132, 868-875
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    Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry

    T. J. Kauppila, N. Talaty, T. Kuuranne, T. Kotiaho, R. Kostiainen and R. G. Cooks, Analyst, 2007, 132, 868
    DOI: 10.1039/B703524A

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