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Issue 8, 2006
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Redistribution of Foscan® from plasma proteins to model membranes

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Abstract

Photodynamic therapy is a comparatively novel modality of tumours treatment that includes simultaneous action of photosensitizers, light and oxygen. Photosensitizer redistribution between plasma proteins and biomembranes define photosensitizers interaction with cells, their intracellular localization and kinetics of sensitizers accumulation in the tumour. Present study investigates the kinetics of Foscan® release from plasma proteins to model membranes using fluorescence resonance energy transfer (FRET) from label, covalently bound to protein, to sensitizer. We have demonstrated very slow kinetics of Foscan® release from protein complexes with rate constants of (1.7 ± 0.1) × 10−3 s−1 for albumin and (1.6 ± 0.3) × 10−4 s−1 for high-density lipoproteins (HDL). Foscan® redistributes by both collision and diffusion-mediated transfer from complexes with HDL, with bimolecular rate constant kout = (8.8 ± 1.4) × 10−2 M−1 s−1. Thermodynamic considerations proposed that sensitizer release from HDL into the aqueous medium is unfavourable and collision mechanism appeared to be a preferred mode of transfer in biological environment. Slow rates of Foscan® redistribution from plasma proteins should be considered while planning dosimetry protocol of Foscan®-PDT.

Graphical abstract: Redistribution of Foscan® from plasma proteins to model membranes

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Publication details

The article was received on 15 Mar 2006, accepted on 13 Jun 2006 and first published on 26 Jun 2006


Article type: Paper
DOI: 10.1039/B603840A
Citation: Photochem. Photobiol. Sci., 2006,5, 770-777
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    Redistribution of Foscan® from plasma proteins to model membranes

    S. Sasnouski, D. Kachatkou, V. Zorin, F. Guillemin and L. Bezdetnaya, Photochem. Photobiol. Sci., 2006, 5, 770
    DOI: 10.1039/B603840A

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