Jump to main content
Jump to site search

Issue 4, 2006
Previous Article Next Article

Photochemically enhanced adenoviral transduction in a multicellular environment

Author affiliations

Abstract

Photochemical internalization (PCI) enhances adenovirus (Ad) transgene expression in a variety of cell lines in vitro. However, measurements of the photochemical effect on transduction in multicellular environments are lacking. In this study, spheroids of DU 145 prostate cancer cells were used as a model to evaluate Ad serotype 5 (Ad5) transduction in a multicellular environment in response to PCI treatment. Furthermore, the Ad5 was coated with poly(2-methyl-acrylic acid 2-[(2-(dimethylamino)-ethyl)-methyl-amino]-ethyl ester) (pDAMA) to evaluate whether physicochemical properties such as charge and size of viral vectors affect transduction of photochemically treated spheroids.

Spheroids incubated with photosensitizer TPPS2a (1 µg ml−1) and infected with adenovirus contained 3-fold higher percentage of reporter gene expressing cells after exposure to blue light (0.42 J cm−2) compared to no light, as analysed by flow cytometry of dissociated spheroids two days after treatment. The cells within the infected spheroids were further divided into three sections corresponding to the interior, intermediate and peripheral layers of the spheroids. This was performed by staining the spheroids with a diffusion-limited dye prior to dissociation. Transduction of cells within photochemically treated and untreated spheroids was heterogeneous, with a radial reduction of transgene expression towards the inner section of the spheroid. The coating of Ad with pDAMA induced up to 2-fold decrease in transduction of cells in the interior section of spheroids compared to uncomplexed Ad, while transduction of the peripheral section remained unchanged. The decrease in transduction could be related to reduced diffusion due to the size of the Ad–pDAMA complexes.

Graphical abstract: Photochemically enhanced adenoviral transduction in a multicellular environment

Back to tab navigation

Publication details

The article was received on 24 Oct 2005, accepted on 23 Feb 2006 and first published on 07 Mar 2006


Article type: Paper
DOI: 10.1039/B515066C
Citation: Photochem. Photobiol. Sci., 2006,5, 411-421
  •   Request permissions

    Photochemically enhanced adenoviral transduction in a multicellular environment

    A. Bonsted, B. Ø. Engesæter, A. Høgset and K. Berg, Photochem. Photobiol. Sci., 2006, 5, 411
    DOI: 10.1039/B515066C

Search articles by author

Spotlight

Advertisements