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Issue 9, 2006
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Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions

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Abstract

FRET-based fluorogenic substrates for lipases and esterases were prepared in four steps from commercially available building blocks. The substrates are pyrenebutyric acid monoesters of aliphatic 1,2-diols bearing a dinitrophenylamino group as a quencher. The most enzyme-reactive substrate is ester 2a. The substrates do not show any measurable background reaction in the absence of enzyme even at pH 11, but react fast and specifically with lipases and esterases. These substrates offer an unprecedented and practical solution to the long-standing problem of a simple yet efficient high-throughput screening tool for lipase activities under basic conditions.

Graphical abstract: Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions

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Publication details

The article was received on 24 Jan 2006, accepted on 16 Feb 2006 and first published on 03 Apr 2006


Article type: Paper
DOI: 10.1039/B601151A
Citation: Org. Biomol. Chem., 2006,4, 1746-1754
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    Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions

    Y. Yang, P. Babiak and J. Reymond, Org. Biomol. Chem., 2006, 4, 1746
    DOI: 10.1039/B601151A

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