Jump to main content
Jump to site search

Issue 10, 2006
Previous Article Next Article

A red-fluorescent substrate microarray for lipase fingerprinting

Author affiliations

Abstract

A lipase substrate microarray was obtained by printing aliphatic C2–C12 monoesters of (5R)- and (5S)-3-(5,6-dihydroxyhexyloxy)benzaldehyde by reductive alkylation on amine-functionalized glass slides coated with bovine serum albumin and a short PEG linker. The microarray features 12 substrates and their 66 possible binary mixtures spotted in a 9 × 36 spot array. Lipase reactions are detected by chemoselective NaIO4-oxidation of the 1,2-diol hydrolysis product to form an aldehyde, which is then tagged with the red-fluorescent dye rhodamine B sulfohydrazide 1. Specific fingerprints are produced by active enzymes. These experiments provide the first example of lipase fingerprinting using microarrays.

Graphical abstract: A red-fluorescent substrate microarray for lipase fingerprinting

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Jun 2006, accepted on 02 Aug 2006 and first published on 24 Aug 2006


Article type: Paper
DOI: 10.1039/B609275F
Citation: Mol. BioSyst., 2006,2, 492-498
  •   Request permissions

    A red-fluorescent substrate microarray for lipase fingerprinting

    J. Grognux and J. Reymond, Mol. BioSyst., 2006, 2, 492
    DOI: 10.1039/B609275F

Search articles by author

Spotlight

Advertisements