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Issue 6-7, 2006
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Disposable real-time microPCR device: lab-on-a-chip at a low cost

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Abstract

We have designed, fabricated and tested a real-time micro polymerase chain reaction (microPCR) system. It consists of a microscope glass cover slip placed on top of a micromachined silicon chip integrated with a heater and a temperature sensor. A single μL of a sample containing DNA was placed on the glass and encapsulated with mineral oil to prevent the evaporation of water, thus forming a virtual reaction chamber (VRC). The PCR chip required half a second to heat up from 72 to 94 °C and two seconds to cool from 94 to 55 °C, corresponding to a cooling rate of −20 K s−1. The real-time PCR yield was determined by a fluorescence method. The melting curve analysis method as well as capillary electrophoresis was performed to determine the purity of the PCR product. As the glass slip is disposable, cross-contamination from sample to sample is eliminated. The total cost of running the PCR is given by the value of the cover slip and its treatment.

Graphical abstract: Disposable real-time microPCR device: lab-on-a-chip at a low cost

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Publication details

The article was received on 21 Feb 2006, accepted on 08 May 2006 and first published on 17 May 2006


Article type: Method
DOI: 10.1039/B605957K
Citation: Mol. BioSyst., 2006,2, 292-298
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    Disposable real-time microPCR device: lab-on-a-chip at a low cost

    P. Neuzil, J. Pipper and T. M. Hsieh, Mol. BioSyst., 2006, 2, 292
    DOI: 10.1039/B605957K

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