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Issue 12, 2006
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A multielectrode microcompartment culture platform for studying signal transduction in the nervous system

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Abstract

This paper describes the design, fabrication, and characterization of a microfabricated compartmented culture system (μ-CCS) useful for electrophysiological signaling studies in cultured neurons. The focus of the paper is the process of interfacing the μ-CCS with cultured neurons and to demonstrate the applicability of the system for biochemical-mediated electrophysiological studies. Moreover, we show that we can record action potentials from cultured neurons through the extracellular compartmented application of elevated levels of K+ ions. Finally, we show that we can isolate the electrophysiological effects of the sodium channel blocker tetrodotoxin in one of the compartments of a two compartment culture while recording electrophysiological data from both compartments.

Graphical abstract: A multielectrode microcompartment culture platform for studying signal transduction in the nervous system

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Publication details

The article was received on 22 May 2006, accepted on 05 Sep 2006 and first published on 21 Sep 2006


Article type: Paper
DOI: 10.1039/B612684G
Citation: Lab Chip, 2006,6, 1530-1536
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    A multielectrode microcompartment culture platform for studying signal transduction in the nervous system

    S. K. Ravula, M. A. McClain, M. S. Wang, J. D. Glass and A. B. Frazier, Lab Chip, 2006, 6, 1530
    DOI: 10.1039/B612684G

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