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Issue 12, 2006
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Open-access microfluidic patch-clamp array with raised lateral cell trapping sites

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Abstract

A novel open-access microfluidic patch-clamp array chip with lateral cell trapping sites raised above the bottom plane of the chip was developed by combining both a microscale soft-lithography and a macroscale polymer fabrication method. This paper demonstrates the capability of using such an open-access fluidic system for patch-clamp measurements. The surface of the open-access patch-clamp sites prepared by the macroscale hole patterning method of soft-state elastic polydimethylsiloxane (PDMS) is examined; the seal resistances are characterized and correlated with the aperture dimensions. Whole cell patch-clamp measurements are carried out with CHO cells expressing Kv2.1 ion channels. Kv2.1 ion channel blocker (TEA) dosage response is characterized and the binding activity is examined. The results demonstrate that the system is capable of performing whole cell measurements and drug profiling in a more efficient manner than the traditional patch-clamp set-up.

Graphical abstract: Open-access microfluidic patch-clamp array with raised lateral cell trapping sites

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Publication details

The article was received on 14 Jun 2006, accepted on 07 Sep 2006 and first published on 27 Sep 2006


Article type: Paper
DOI: 10.1039/B608439G
Citation: Lab Chip, 2006,6, 1510-1515
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    Open-access microfluidic patch-clamp array with raised lateral cell trapping sites

    A. Y. Lau, P. J. Hung, A. R. Wu and L. P. Lee, Lab Chip, 2006, 6, 1510
    DOI: 10.1039/B608439G

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