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Issue 10, 2006
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A bio-barcode assay for on-chip attomolar-sensitivity protein detection

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Abstract

Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles—a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 µL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.

Graphical abstract: A bio-barcode assay for on-chip attomolar-sensitivity protein detection

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Publication details

The article was received on 04 May 2006, accepted on 28 Jul 2006 and first published on 15 Aug 2006


Article type: Paper
DOI: 10.1039/B606294F
Citation: Lab Chip, 2006,6, 1293-1299
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    A bio-barcode assay for on-chip attomolar-sensitivity protein detection

    E. D. Goluch, J. Nam, D. G. Georganopoulou, T. N. Chiesl, K. A. Shaikh, K. S. Ryu, A. E. Barron, C. A. Mirkin and C. Liu, Lab Chip, 2006, 6, 1293
    DOI: 10.1039/B606294F

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