Jump to main content
Jump to site search

Issue 8, 2006
Previous Article Next Article

A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells

Author affiliations

Abstract

The importance of research involving non-adherent cell lines, primary cells and blood cells is generally undisputed. However, the task of investigating the complexity and heterogeneity of these cells calls for their long-run monitoring at a single-cell resolution. Such a capability is currently unavailable without having to use disruptive cell tethering. The present Cell Retainer (CR) concept enables high-content correlative multi-parametric measurements, from the functional to molecular level, of the same living individual non-adherent cells within a population. Thereby, despite extensive long-term bio-manipulations, the cells preserve their identity without tethering. Several exemplary experiments, using a microscope-slide-based version of the CR, are presented, which could not be performed by other state of the art methods.

Graphical abstract: A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Mar 2006, accepted on 17 May 2006 and first published on 02 Jun 2006


Article type: Paper
DOI: 10.1039/B603961H
Citation: Lab Chip, 2006,6, 995-1000
  •   Request permissions

    A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells

    M. Deutsch, A. Deutsch, O. Shirihai, I. Hurevich, E. Afrimzon, Y. Shafran and N. Zurgil, Lab Chip, 2006, 6, 995
    DOI: 10.1039/B603961H

Search articles by author

Spotlight

Advertisements