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Issue 9, 2006
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Two-dimensional impedance imaging of cell migration and epithelial stratification

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Abstract

We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 µm × 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validated. The system uses an Agilent 4294A impedance analyser combined with a front-end amplifier for the impedance measurements. Human epithelial stem cells (YF 29) were grown on the device surface. Cell migration speeds of 300 nm min−1 following a “scratch” wound closure assay could be established. Using a commercial software developed for geophysical prospecting, we could generate impedance tomography images at 10 kHz revealing cell migration, increase of epithelial thickness and changes in tissue resistivity over a time course of several days.

Graphical abstract: Two-dimensional impedance imaging of cell migration and epithelial stratification

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Publication details

The article was received on 15 Mar 2006, accepted on 29 Jun 2006 and first published on 03 Aug 2006


Article type: Paper
DOI: 10.1039/B603856E
Citation: Lab Chip, 2006,6, 1155-1162
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    Two-dimensional impedance imaging of cell migration and epithelial stratification

    P. Linderholm, T. Braschler, J. Vannod, Y. Barrandon, M. Brouard and P. Renaud, Lab Chip, 2006, 6, 1155
    DOI: 10.1039/B603856E

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