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Issue 6, 2006
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Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap

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Abstract

Biomimetic heterogeneous patterning of hepatic and endothelial cells, which start from randomly distributed cells inside the microfluidic chamber, via the chip design of enhanced field-induced dielectrophoresis (DEP) trap is demonstrated and reported in this paper. The concentric-stellate-tip electrode array design in this chip generates radial-pattern electric fields for the DEP manipulation of the live liver cells. By constructing the geometric shape and the distribution of stellate tips, the DEP electrodes enhance the desired spatial electric-field gradients to guide and snare individual cells to form the desired biomimetic pattern. With this proposed microfluidic chip design, the original randomly distributed hepatocytes inside the microfluidic chamber can be manipulated in parallel and align into the desired pearl-chain array pattern. This radial pattern mimics the lobular morphology of real liver tissue. The endothelial cells, then, are snared into the additional pearl-chain array and settle at the space in-between the previous hepatic pearl-chain array. By this cell-lab chip, we demonstrate the in vitro reconstruction of the heterogeneous lobule-mimetic radial pattern with good cell viability after cell patterning. This work reports the rapid in-parallel patterning of the dual types of live liver cells via the enhanced DEP trap inside the microfluidic chip.

Graphical abstract: Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap

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Publication details

The article was received on 10 Feb 2006, accepted on 13 Apr 2006 and first published on 03 May 2006


Article type: Paper
DOI: 10.1039/B602036D
Citation: Lab Chip, 2006,6, 724-734
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    Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap

    C. Ho, R. Lin, W. Chang, H. Chang and C. Liu, Lab Chip, 2006, 6, 724
    DOI: 10.1039/B602036D

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