Jump to main content
Jump to site search

Issue 9, 2006
Previous Article Next Article

An integrated digital microfluidic chip for multiplexed proteomic sample preparation and analysis by MALDI-MS

Author affiliations

Abstract

To realize multiplexed sample preparation on a digital microfluidic chip for high-throughput Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS), several fluidic functions need to be integrated. These include the generation of multiple droplets from a reservoir and parallel in-line sample purification. In this paper, we develop two critical new functions in handling protein solutions and standard proteomic reagents with electrowetting-on-dielectric (EWOD) actuation, leading to an integrated chip for multiplexed sample preparation for MALDI-MS. The first is a voltage sequence designed to generate a series of droplets from each of the three reservoirs—proteomic sample, rinsing fluid, and MALDI reagents. It is the first time that proteomic reagents have been dispensed using EWOD in an air (as opposed to oil) environment. The second is a box-in-box electrode pattern developed to allow droplet passing over dried sample spots, making the process of in-line sample purification robust for parallel processing. As a result, parallel processing of multiple sample droplets is demonstrated on the integrated EWOD-MALDI-MS chip, an important step towards high-throughput MALDI-MS. The MS results, collected directly from the integrated devices, are of good quality, suggesting that the tedious process of sample preparation can be automated on-chip for MALDI-MS applications as well as other high-throughput proteomics applications.

Graphical abstract: An integrated digital microfluidic chip for multiplexed proteomic sample preparation and analysis by MALDI-MS

Back to tab navigation

Publication details

The article was received on 09 Feb 2006, accepted on 16 Jun 2006 and first published on 24 Jul 2006


Article type: Paper
DOI: 10.1039/B601954D
Citation: Lab Chip, 2006,6, 1213-1219
  •   Request permissions

    An integrated digital microfluidic chip for multiplexed proteomic sample preparation and analysis by MALDI-MS

    H. Moon, A. R. Wheeler, R. L. Garrell, J. A. Loo and C. “. Kim, Lab Chip, 2006, 6, 1213
    DOI: 10.1039/B601954D

Search articles by author

Spotlight

Advertisements