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Issue 7, 2006
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Microchip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification

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Abstract

Optimal detection of a pathogen present in biological samples depends on the ability to extract DNA molecules rapidly and efficiently. In this paper, we report a novel method for efficient DNA extraction and subsequent real-time detection in a single microchip by combining laser irradiation and magnetic beads. By using a 808 nm laser and carboxyl-terminated magnetic beads, we demonstrate that a single pulse of 40 seconds lysed pathogens including E. coli and Gram-positive bacterial cells as well as the hepatitis B virus mixed with human serum. We further demonstrate that the real-time pathogen detection was performed with pre-mixed PCR reagents in a real-time PCR machine using the same microchip, after laser irradiation in a hand-held device equipped with a small laser diode. These results suggest that the new sample preparation method is well suited to be integrated into lab-on-a-chip application of the pathogen detection system.

Graphical abstract: Microchip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification

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Publication details

The article was received on 08 Nov 2005, accepted on 06 Apr 2006 and first published on 02 May 2006


Article type: Paper
DOI: 10.1039/B515876A
Citation: Lab Chip, 2006,6, 886-895
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    Microchip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification

    J. Lee, K. H. Cheong, N. Huh, S. Kim, J. Choi and C. Ko, Lab Chip, 2006, 6, 886
    DOI: 10.1039/B515876A

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