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Issue 2, 2006
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Characterization of a microfluidic dispensing system for localised stimulation of cellular networks

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Abstract

We present a 3-D microfluidic device designed for localized drug delivery to cellular networks. The device features a flow cell comprising a main channel for nutrient delivery as well as multiple channels for drug delivery. This device is one key component of a larger, fully integrated system now under development, based upon a microelectrode array (MEA) with on-chip CMOS circuitry for recording and stimulation of electrogenic cells (e.g. neurons, cardiomyocytes). As a critical system unit, the microfluidics must be carefully designed and characterized to ensure that candidate drugs are delivered to specific regions of the culture at known concentrations. Furthermore, microfluidic design and functionality is dictated by the size, geometry, and material/electrical characteristics of the CMOS MEA. Therefore, this paper reports on the design considerations and fabrication of the flow cell, including theoretical and experimental analysis of the mass transfer properties of the nutrient and drug flows, which are in good agreement with one another. To demonstrate proof of concept, the flow cell was mounted on a dummy CMOS chip, which had been plated with HL-1 cardiomyocytes. A test chemical compound was delivered to the cell culture in a spatially resolved manner. Envisioned applications of this stand-alone system include simultaneous toxicological testing of multiple compounds and chemical stimulation of natural neural networks for neuroscience investigations

Graphical abstract: Characterization of a microfluidic dispensing system for localised stimulation of cellular networks

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Publication details

The article was received on 18 Aug 2005, accepted on 06 Dec 2005 and first published on 04 Jan 2006


Article type: Paper
DOI: 10.1039/B511768B
Citation: Lab Chip, 2006,6, 218-229
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    Characterization of a microfluidic dispensing system for localised stimulation of cellular networks

    T. Kraus, E. Verpoorte, V. Linder, W. Franks, A. Hierlemann, F. Heer, S. Hafizovic, T. Fujii, N. F. de Rooij and S. Koster, Lab Chip, 2006, 6, 218
    DOI: 10.1039/B511768B

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