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Issue 11, 2006
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T cell chemotaxis in a simple microfluidic device

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Abstract

This paper describes the use of a simple microfluidic device for studying T cell chemotaxis. The microfluidic device is fabricated in poly(dimethylsiloxane) (PDMS) using soft-lithography and consists of a “Y” type fluidic channel. Solutions are infused into the device by syringe pumps and generate a concentration gradient in the channel by diffusion. We show that the experimentally measured gradient profiles agree nicely with theoretical predictions and the gradient is stable in the observation region for cell migration. Using this device, we demonstrate robust chemotaxis of human T cells in response to single and competing gradients of chemokine CCL19 and CXCL12. Because of the simplicity of the device, it can flexibly control gradient generation in space and time, and would allow generation of multiple gradient conditions in a single chip for highly parallel chemotaxis experimentation. Visualization of T cell chemotaxis has previously been limited to studies in 3D matrices or under agarose assays, which do not allow precise control or variation in conditions. Acknowledging the importance of lymphocyte homing in the adaptive immune response, the ability to study T cell chemotaxis in microfluidic devices offers a new approach for investigating lymphocyte migration and chemotaxis in vitro.

Graphical abstract: T cell chemotaxis in a simple microfluidic device

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Publication details

The article was received on 23 May 2006, accepted on 17 Aug 2006 and first published on 04 Sep 2006


Article type: Paper
DOI: 10.1039/B607071J
Citation: Lab Chip, 2006,6, 1462-1469
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    T cell chemotaxis in a simple microfluidic device

    F. Lin and E. C. Butcher, Lab Chip, 2006, 6, 1462
    DOI: 10.1039/B607071J

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