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Issue 8, 2006
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Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform

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Abstract

We present a novel microfluidic concept to enable a fast colorimetric alcohol assay from a single droplet of whole blood. The reduced turn-around time of 150 seconds is, on the one hand, achieved by a full process integration including metering, mixing with reagents, and sedimentation of cellular constituents. On the other hand, our novel total internal reflection (TIR) scheme allows to monitor the increase of the absorbance values in real-time. Thus, the saturation values can be predicted accurately based on an extrapolation of real-time measurements acquired during a 100 second initial period of rotation. Additionally, we present a metering structure to define nanolitre sample volumes at a coefficient of variation (CV) below 5%.

Graphical abstract: Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform

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Publication details

The article was received on 17 May 2006, accepted on 17 May 2006 and first published on 05 Jun 2006


Article type: Paper
DOI: 10.1039/B607051P
Citation: Lab Chip, 2006,6, 1040-1044
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    Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform

    J. Steigert, M. Grumann, T. Brenner, L. Riegger, J. Harter, R. Zengerle and J. Ducrée, Lab Chip, 2006, 6, 1040
    DOI: 10.1039/B607051P

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