Issue 10, 2006
From the journal:

Lab on a Chip

A bio-barcode assay for on-chip attomolar-sensitivity protein detection†

Edgar D. Goluch,a   Jwa-Min Nam,‡b   Dimitra G. Georganopoulou,b   Thomas N. Chiesl,c   Kashan A. Shaikh,a   Kee S. Ryu,a   Annelise E. Barron,c   Chad A. Mirkinb  and  Chang Liu*a  

* Corresponding authors

a Micro and Nanotechnology Laboratory, Bioengineering Department, University of Illinois at Urbana-Champaign, 208 N. Wright Street, Urbana, IL 61801, USA
E-mail: changliu@uiuc.edu
Fax: 1-217-244-6375
Tel: 1-217-333-4051

b Department of Chemistry and Institute of Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA

c Department of Chemical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA

Abstract

Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles—a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 µL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.

Graphical abstract: A bio-barcode assay for on-chip attomolar-sensitivity protein detection

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Article information

DOI
https://doi.org/10.1039/B606294F
Article type
Paper
Submitted
04 May 2006
Accepted
28 Jul 2006
First published
15 Aug 2006

Lab Chip, 2006,6, 1293-1299

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A bio-barcode assay for on-chip attomolar-sensitivity protein detection

E. D. Goluch, J. Nam, D. G. Georganopoulou, T. N. Chiesl, K. A. Shaikh, K. S. Ryu, A. E. Barron, C. A. Mirkin and C. Liu, Lab Chip, 2006, 6, 1293 DOI: 10.1039/B606294F

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