Jump to main content
Jump to site search

Issue 7, 2006
Previous Article Next Article

Microchemostat—microbial continuous culture in a polymer-based, instrumented microbioreactor

Author affiliations

Abstract

In a chemostat, microbial cells reach a steady state condition at which cell biomass production, substrates and the product concentrations remain constant. These features make continuous culture a unique and powerful tool for biological and physiological research. We present a polymer-based microbioreactor system integrated with optical density (OD), pH, and dissolved oxygen (DO) real-time measurements for continuous cultivation of microbial cells. Escherichia coli (E. coli) cells are continuously cultured in a 150 µL, membrane-aerated, well-mixed microbioreactor fed by a pressure-driven flow of fresh medium through a microchannel. Chemotaxisial back growth of bacterial cells into the medium feed channel is prevented by local heating. Using poly(ethylene glycol) (PEG)-grafted poly(acrylic acid) (PAA) copolymer films, the inner surfaces of poly(methyl methacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) of the microbioreactor are modified to generate bio-inert surfaces resistant to non-specific protein adsorption and cell adhesion. The modified surfaces of microbioreactor effectively reduce wall growth of E. coli for a prolonged period of cultivation. Steady state conditions at different dilution rates are demonstrated and characterized by steady OD, pH, and DO levels.

Graphical abstract: Microchemostat—microbial continuous culture in a polymer-based, instrumented microbioreactor

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 05 Jan 2006, accepted on 04 Apr 2006 and first published on 20 Apr 2006


Article type: Paper
DOI: 10.1039/B518396K
Citation: Lab Chip, 2006,6, 906-913
  •   Request permissions

    Microchemostat—microbial continuous culture in a polymer-based, instrumented microbioreactor

    Z. Zhang, P. Boccazzi, H. Choi, G. Perozziello, A. J. Sinskey and K. F. Jensen, Lab Chip, 2006, 6, 906
    DOI: 10.1039/B518396K

Search articles by author