Issue 11, 2006

Investigating the formation of an Sb(v)–citrate complex by HPLC-ICP-MS and HPLC-ES-MS(/MS)

Abstract

Complexation of Sb(V) with citrate has recently been shown to be of relevance in food chemistry. Here we characterize the products of the reaction of inorganic Sb(V) with citric acid by use of various chromatographic methods coupled on-line with inductively coupled plasma mass spectrometry (ICP-MS) and electrospray (ES)-MS(/MS). By use of HPLC-ICP-MS, estimates for the conditional formation constants are obtained for the main product of the reaction, the 1 ∶ 1 Sb(V)–citrate complex, present in reaction systems of pH 3, 5 and 7 (log Kcond = 4.6, 3.75 and 1.76, respectively). The physiological relevance of an Sb(V)–citrate complex was studied by use of HPLC-ICP-MS and ES-MS by evaluating: (1) the competition of Sb(V) and Al(III) for binding to citrate; (2) the competition of citrate and adenosine for binding to Sb(V); (3) the rate of reduction of inorganic Sb(V) and Sb(V)–citrate complex in the presence of L-cysteine; and (4) the formation of the Sb(V)–citrate complex in urine samples spiked with inorganic Sb(V). All results of this study suggest that the Sb(V)–citrate complex may be of physiological relevance, especially in the acidic cell compartments. A highly selective and sensitive detection method (SRM-ES-MS/MS) was developed for the screening of Sb(V)–citrate potentially present in biological samples.

Graphical abstract: Investigating the formation of an Sb(v)–citrate complex by HPLC-ICP-MS and HPLC-ES-MS(/MS)

Article information

Article type
Paper
Submitted
30 May 2006
Accepted
23 Aug 2006
First published
18 Sep 2006

J. Anal. At. Spectrom., 2006,21, 1240-1248

Investigating the formation of an Sb(V)–citrate complex by HPLC-ICP-MS and HPLC-ES-MS(/MS)

H. R. Hansen and S. A. Pergantis, J. Anal. At. Spectrom., 2006, 21, 1240 DOI: 10.1039/B607621A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements