Issue 3, 2006
From the journal:

Chemical Communications

Intein-mediated in vitro synthesis of lipidated Ras proteins†

D. Gottlieb,ab   C. Grunwald,c   C. Nowak,c   J. Kuhlmann*c  and  H. Waldmann*ab  

* Corresponding authors

a Max Planck Institute of Molecular Physiology, Otto-Hahn-Str. 11, Dortmund, Germany
E-mail: herbert.waldmann@mpi-dortmund.mpg.de
Fax: (+49)231 133 2499
Tel: (+49) 133 2400

b Department of Chemical Biology, University of Dortmund, Otto-Hahn-Str. 6, Dortmund, Germany
E-mail: herbert.waldmann@mpi-dortmund.mpg.de
Fax: (+49)231 133 2499
Tel: (+49) 133 2400

c Max Planck Institute of Molecular Physiology, Department of Structural Biology, Otto-Hahn-Str. 11, Dortmund, Germany
E-mail: juergen.kuhlmann@mpi-dortmund.mpg.de
Fax: (+49) 133 2199
Tel: (+49) 133 2104

Abstract

Fully functional lipid-modified Ras proteins suitable for the study of Ras–membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means of Expressed Protein Ligation.

Graphical abstract: Intein-mediated in vitro synthesis of lipidated Ras proteins

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Article information

DOI
https://doi.org/10.1039/B511736D
Article type
Communication
Submitted
18 Aug 2005
Accepted
10 Nov 2005
First published
28 Nov 2005

Chem. Commun., 2006, 260-262

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Intein-mediated in vitro synthesis of lipidated Ras proteins

D. Gottlieb, C. Grunwald, C. Nowak, J. Kuhlmann and H. Waldmann, Chem. Commun., 2006, 260 DOI: 10.1039/B511736D

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