Jump to main content
Jump to site search

Issue 5, 2006
Previous Article Next Article

Analytical performances of FT-IR spectrometry and imaging for concentration measurements within biological fluids, cells, and tissues

Author affiliations

Abstract

FT-IR spectrometry has proved to be a useful tool for determining a series of plasma molecular concentrations. Dedicated experiments were first performed to test the analytical performance that could be obtained by FT-IR spectrometry using a synthesized N3-peptide exhibiting a –N3 absorption centered at 2110 cm−1, a spectral region where no organic material of biological samples absorbs. Further, we investigated whether this technology was able to allow quantification of metabolic parameters (glucose and lactic acid) within plasma, cells, and tissues as an alternative method to the “classical” biochemical approaches, which require sophisticated biological material treatment and expensive reagents. For this purpose we used a series of plasma samples to determine glucose and lactic acid concentrations, which are common markers of cancer growth. We compared the results of the main spectral data treatments commonly achieved for FT-IR data analysis, such as univariate (Beer–Lambert) or multivariate (PLS) calibrations, as well as the deconvolution of the spectral interval of interest (1200–900 cm−1). No significant differences were found regarding the analytical performances of these methods. Spectral deconvolution was finally undertaken on cultured and on xenografted cells (U87 glial cells implied in human gliomas) to determine glucose and lactic acid concentrations. In this case, qualification was allowed by FT-IR imaging on the cellular models since biochemical approaches are not efficient to reach metabolic concentrations at the cellular level while keeping tissue organization.

Graphical abstract: Analytical performances of FT-IR spectrometry and imaging for concentration measurements within biological fluids, cells, and tissues

Back to tab navigation

Publication details

The article was received on 21 Dec 2005, accepted on 21 Mar 2006 and first published on 31 Mar 2006


Article type: Paper
DOI: 10.1039/B518076G
Citation: Analyst, 2006,131, 640-647
  •   Request permissions

    Analytical performances of FT-IR spectrometry and imaging for concentration measurements within biological fluids, cells, and tissues

    C. Petibois, K. Gionnet, M. Gonçalves, A. Perromat, M. Moenner and G. Déléris, Analyst, 2006, 131, 640
    DOI: 10.1039/B518076G

Search articles by author

Spotlight

Advertisements