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Issue 12, 2005
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Photosystem I: a search for green plant trimers

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Abstract

Recent blue-native gel electrophoresis studies gave evidence for the existence of dimeric and trimeric PSI complexes in green plants. We used single particle electron microscopy to investigate all the larger particles from the thylakoid membrane of pea (Pisum sativum var. Charmette). Peak fractions with monomeric, dimeric and trimeric Photosystem I were obtained after solubilization with digitonin and size-exclusion chromatography. The analysis showed that only a few percent of dimers and trimers were present. In the best resolved trimers some of the monomers were oriented upside down. Many classes were fuzzy, indicating a non-specific or flexible orientation. From these results we conclude that the green plant PSI is monomeric within the green plant membrane.

Graphical abstract: Photosystem I: a search for green plant trimers

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Publication details

The article was received on 20 Apr 2005, accepted on 23 Jun 2005 and first published on 25 Jul 2005


Article type: Technical Note
DOI: 10.1039/B505519A
Citation: Photochem. Photobiol. Sci., 2005,4, 1091-1094
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    Photosystem I: a search for green plant trimers

    R. Kouřil, N. van Oosterwijk, A. E. Yakushevska and E. J. Boekema, Photochem. Photobiol. Sci., 2005, 4, 1091
    DOI: 10.1039/B505519A

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