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Issue 8, 2005
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Preparation of sialylated oligosaccharides employing recombinant trans-sialidase from Trypanosoma cruzi

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Abstract

Terminally sialylated oligosaccharides were synthesised employing recombinant trans-sialidase from Trypanosoma cruzi. Regio- and stereoselectively Sia-α(2–3)-Gal-βR derivatives could be obtained in respectable yields, using combined chemical and enzymatic methodologies. An array of different disaccharide precursors such as Gal-β(1–3)-GalNAc-αSer/Thr, lactosides and lactosamide derivatives were sialylated and successfully purified by facile isolation procedures. Depending on the acceptor structure isolated, yields for trans-sialylation products were between 20 and 60%.

Graphical abstract: Preparation of sialylated oligosaccharides employing recombinant trans-sialidase from Trypanosoma cruzi

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Publication details

The article was received on 05 Jan 2005, accepted on 10 Feb 2005 and first published on 22 Mar 2005


Article type: Paper
DOI: 10.1039/B500042D
Citation: Org. Biomol. Chem., 2005,3, 1551-1556
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    Preparation of sialylated oligosaccharides employing recombinant trans-sialidase from Trypanosoma cruzi

    B. Neubacher, D. Schmidt, P. Ziegelmüller and J. Thiem, Org. Biomol. Chem., 2005, 3, 1551
    DOI: 10.1039/B500042D

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