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Issue 5-6, 2005
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Glucose biosensors as models for the development of advanced protein-based biosensors

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Abstract

Glucose sensing is used as a model to explore the advantages and problems deriving from the use of either enzymes or sugar binding proteins to develop stable fluorescence biosensors. We report on a novel approach to address the problem of substrate consumption by sensors based on enzymes, namely the utilization of apo-enzymes as non-active forms of the protein which are still able to bind the substrate/ligand. We also review studies in which derivatization of a naturally thermostable sugar-binding protein with a fluorescent probe allows quantitative monitoring of glucose binding even after immobilization on a solid support.

Graphical abstract: Glucose biosensors as models for the development of advanced protein-based biosensors

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Publication details

The article was received on 21 Sep 2005, accepted on 20 Oct 2005 and first published on 04 Nov 2005


Article type: Review Article
DOI: 10.1039/B513385H
Citation: Mol. BioSyst., 2005,1, 354-362
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    Glucose biosensors as models for the development of advanced protein-based biosensors

    M. Staiano, P. Bazzicalupo, M. Rossi and S. D'Auria, Mol. BioSyst., 2005, 1, 354
    DOI: 10.1039/B513385H

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