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Issue 12, 2005
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The autofluorescence of plastic materials and chips measured under laser irradiation

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Abstract

Plastic materials have the potential to substitute for glass substrates used in microfluidic and μTAS systems adding flexibility in materials' choices. Optical quality plastic materials with a low autofluorescence are crucial for optimal detection by fluorescence and laser induced fluorescence techniques. This paper summarizes a series of optical investigations on commercially available plastic chip materials (PMMA, COC, PC, PDMS) and chips made from those materials. Intrinsic optical constants of plastic materials—refractive index for bulk materials—determined by spectroscopic ellipsometry and transmission spectroscopy in the visible range are presented. The laser-induced autofluorescence of materials and chips was assessed at four laser wavelengths, namely, 403, 488, 532 and 633 nm. Considerable bleaching of the autofluorescence was observed under continuous laser illumination. Overall, the longer wavelength laser excitation sources yielded less autofluorescence. PDMS exhibited the least autofluorescence and was comparable to BoroFloat glass. In all cases, chips exhibited slightly higher autofluorescence than the raw plastic materials from which they had been made.

Graphical abstract: The autofluorescence of plastic materials and chips measured under laser irradiation

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Publication details

The article was received on 13 Jun 2005, accepted on 10 Oct 2005 and first published on 01 Nov 2005


Article type: Paper
DOI: 10.1039/B508288A
Citation: Lab Chip, 2005,5, 1348-1354
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    The autofluorescence of plastic materials and chips measured under laser irradiation

    A. Piruska, I. Nikcevic, S. H. Lee, C. Ahn, W. R. Heineman, P. A. Limbach and C. J. Seliskar, Lab Chip, 2005, 5, 1348
    DOI: 10.1039/B508288A

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