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Issue 11, 2005
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A lab-on-a-chip for spectrophotometric analysis of biological fluids

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Abstract

This paper reports a lab-on-a-chip for application in clinical analysis, especially in the spectrophotometric analysis of biological fluids. It is composed of three parts: (1) a microfluidic system die containing the microchannels fabricated using SU-8 techniques; (2) an optical filtering system based on highly selective Fabry–Perot optical resonators using a stack of CMOS process compatible thin-film layers; (3) a detection and readout system fabricated in a CMOS microelectronic process. The system enables low-cost and selective measurement of the concentration of several biomolecules in biological fluids. Operation is based on optical absorption in a well-defined part of the visible spectrum, defined by the reaction of a specific reagent with a specific biomolecule. Signals proportional to the intensity of the light transmitted through the biological fluid are available at the output in the form of bit streams, which allows simple computer interfacing. Moreover, the optical filtering system enables the measurement using white light illumination, thus avoiding the use of a wavelength dependent light source. This characteristic makes the lab-on-a-chip portable and ensures that the analysis can be performed at any location with instantaneous results, without the use of complex and expensive analysis systems. The quantitative measurement of uric acid and total protein in urine is demonstrated.

Graphical abstract: A lab-on-a-chip for spectrophotometric analysis of biological fluids

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Publication details

The article was received on 13 May 2005, accepted on 18 Aug 2005 and first published on 22 Sep 2005


Article type: Paper
DOI: 10.1039/B506817G
Citation: Lab Chip, 2005,5, 1303-1309
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    A lab-on-a-chip for spectrophotometric analysis of biological fluids

    G. Minas, R. F. Wolffenbuttel and J. H. Correia, Lab Chip, 2005, 5, 1303
    DOI: 10.1039/B506817G

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