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Issue 10, 2005
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Integrated microsystem for dielectrophoretic cell concentration and genetic detection

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Abstract

We have directly integrated a continuous-flow, electrokinetic method of bacterial cell concentration with room temperature, sequence-specific genetic detection. The system we have developed traps cells from a continuous-flow sample stream via dielectrophoresis, providing a 160-fold increase in cell concentration. PDMS microvalves then define a 100 nL volume around the trapped cells to which cell lysis buffer and genetic detection components are introduced. Direct, optical detection of Escherichia coli MC1061 cells is then accomplished via the sequence-specific hybridization of an rRNA-directed optical molecular beacon. This integrated microsystem is capable of sequence-specific genetic detection of 25 cells within 30 min.

Graphical abstract: Integrated microsystem for dielectrophoretic cell concentration and genetic detection

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Publication details

The article was received on 27 Apr 2005, accepted on 16 Aug 2005 and first published on 31 Aug 2005


Article type: Paper
DOI: 10.1039/B505915A
Citation: Lab Chip, 2005,5, 1053-1058
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    Integrated microsystem for dielectrophoretic cell concentration and genetic detection

    E. T. Lagally, S. Lee and H. T. Soh, Lab Chip, 2005, 5, 1053
    DOI: 10.1039/B505915A

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