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Issue 8, 2005
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World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays

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Abstract

We report a practical world-to-chip microfluidic interfacing method with built-in valves suitable for microscale multichamber chip-based assays. One of the primary challenges associated with the successful commercialization of fully integrated microfluidic systems has been the lack of reliable world-to-chip microfluidic interconnections. After sample loading and sealing, leakage tests were conducted at 100 °C for 30 min and no detectable leakage flows were found during the test for 100 microchambers. To demonstrate the utility of our world-to-chip microfluidic interface, we designed a microscale PCR chip with four chambers and performed PCR assays. The PCR results yielded a 100% success rate with no contamination or leakage failures. In conclusion, we have introduced a simple and inexpensive microfluidic interfacing system for both sample loading and sealing with no dead volume, no leakage flow and biochemical compatibility.

Graphical abstract: World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays

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Publication details

The article was received on 08 Mar 2005, accepted on 16 May 2005 and first published on 23 Jun 2005


Article type: Paper
DOI: 10.1039/B503437J
Citation: Lab Chip, 2005,5, 845-850
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    World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays

    K. W. Oh, C. Park, K. Namkoong, J. Kim, K. Ock, S. Kim, Y. Kim, Y. Cho and C. Ko, Lab Chip, 2005, 5, 845
    DOI: 10.1039/B503437J

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