Jump to main content
Jump to site search

Issue 8, 2005
Previous Article Next Article

World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays

Author affiliations

Abstract

We report a practical world-to-chip microfluidic interfacing method with built-in valves suitable for microscale multichamber chip-based assays. One of the primary challenges associated with the successful commercialization of fully integrated microfluidic systems has been the lack of reliable world-to-chip microfluidic interconnections. After sample loading and sealing, leakage tests were conducted at 100 °C for 30 min and no detectable leakage flows were found during the test for 100 microchambers. To demonstrate the utility of our world-to-chip microfluidic interface, we designed a microscale PCR chip with four chambers and performed PCR assays. The PCR results yielded a 100% success rate with no contamination or leakage failures. In conclusion, we have introduced a simple and inexpensive microfluidic interfacing system for both sample loading and sealing with no dead volume, no leakage flow and biochemical compatibility.

Graphical abstract: World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays

Back to tab navigation

Publication details

The article was received on 08 Mar 2005, accepted on 16 May 2005 and first published on 23 Jun 2005


Article type: Paper
DOI: 10.1039/B503437J
Citation: Lab Chip, 2005,5, 845-850
  •   Request permissions

    World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays

    K. W. Oh, C. Park, K. Namkoong, J. Kim, K. Ock, S. Kim, Y. Kim, Y. Cho and C. Ko, Lab Chip, 2005, 5, 845
    DOI: 10.1039/B503437J

Search articles by author

Spotlight

Advertisements