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Issue 4, 2005
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Laser induced disruption of bacterial spores on a microchip

Oliver Hofmann,a  Kirk Murray,b  Alan-Shaun Wilkinson,c  Timothy Coxc  and  Andreas Manz*d 
Author affiliations

* Corresponding authors

a Department of Chemistry, Imperial College London, London, UK

b Dstl, Detection Department, Porton Down, Salisbury, UK

c QinetiQ, Malvern Technology Centre, Malvern, UK

d ISAS (Institute of Spectrochemistry and Applied Spectroscopy), Germany
E-mail: a.manz@isas-dortmund.de
Fax: +49 231 1392 300
Tel: +49 231 1392 103/102

Abstract

We report on the development of a laser based spore disruption method. Bacillus globigii spores were mixed with a laser light absorbing matrix and co-crystallized into 200-µm-wide and 20-µm-deep nanovials formed in a polydimethylsiloxane (PDMS) target plate. Surface tension effects were exploited to effect up to 125-fold spore enrichment. When the target zones were illuminated at atmospheric pressure with pulsed UV-laser light at fluences below 20 mJ cm−2 a change in spore morphology was observed within seconds. Post illumination PCR analysis suggests the release of endogenous DNA indicative of spore disruption. For laser fluences above 20 mJ cm−2, desorption of spores and fragments was also observed even without a matrix being employed. Desorbed material was collected in a PDMS flowcell attached to the target plate during laser illumination. This opens up a route towards the direct extraction of released DNA in an integrated spore disruption–PCR amplification microchip device.

Graphical abstract: Laser induced disruption of bacterial spores on a microchip

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Publication details

The article was received on 13 Dec 2004, accepted on 07 Feb 2005 and first published on 23 Feb 2005


Article type: Communication
DOI: 10.1039/B418663J
Citation: Lab Chip, 2005,5, 374-377
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    Laser induced disruption of bacterial spores on a microchip

    O. Hofmann, K. Murray, A. Wilkinson, T. Cox and A. Manz, Lab Chip, 2005, 5, 374
    DOI: 10.1039/B418663J

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