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Issue 3, 2005
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Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

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Abstract

A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucidation of glycoconjugates originating from human body fluids and tissues such as a glycopeptide mixture from normal human urine and an isolated and purified GT1 ganglioside fraction from normal adult human brain. The optimization procedure required by each glycoconjugate category is described and the advantages of the system in terms of flexibility and adaptability to QTOF MS, stability of the ESI MS signal, carbohydrate ionization and sequencing, sensitivity, speed of analysis and sample consumption are discussed.

Graphical abstract: Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

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Publication details

The article was received on 01 Sep 2004, accepted on 09 Nov 2004 and first published on 22 Dec 2004


Article type: Paper
DOI: 10.1039/B413282C
Citation: Lab Chip, 2005,5, 298-307
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    Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

    A. D. Zamfir, N. Lion, Ž. Vukelić, L. Bindila, J. Rossier, H. H. Girault and J. Peter-Katalinić, Lab Chip, 2005, 5, 298
    DOI: 10.1039/B413282C

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