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Issue 1, 2005
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Differentiation-on-a-chip: A microfluidic platform for long-term cell culture studies

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Abstract

Here we demonstrate a microfluidic perfusion system suitable for a long-term (>2 week) culture of muscle cells spanning the whole process of differentiation from myoblasts to myotubes. Cell-adhesive surface microdomains alternating with a robust cell-repellent coating mimic in vivo spatial cues for muscle cell assembly and allow for confining the fusion of myoblasts into aligned, isolated multinucleated myotubes. The microfluidic system provides accurate control of the perfusion rates and biochemical composition of the environment surrounding the cells. Comparing muscle cell-specific differentiation markers and the timing of fusion, we observed no differences in differentiation between microfluidic and traditional cultures. All differentiation assays were fully microfluidic, i.e. they were performed by sequentially changing the fluids in the microchannels. By delivering fluorescent markers using heterogeneous laminar flows, it was possible to confine a membrane receptor labeling assay to a region smaller than a myotube. Our method can serve as an improved in vitro model for studying muscle cell differentiation and for characterizing extracellular molecules and mechanisms involved in neuromuscular differentiation.

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Publication details

The article was received on 16 Apr 2004, accepted on 17 Jun 2004 and first published on 26 Jul 2004


Article type: Communication
DOI: 10.1039/B405719H
Citation: Lab Chip, 2005,5, 14-19
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    Differentiation-on-a-chip: A microfluidic platform for long-term cell culture studies

    A. Tourovskaia, X. Figueroa-Masot and A. Folch, Lab Chip, 2005, 5, 14
    DOI: 10.1039/B405719H

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