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Issue 5, 2005
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Sample introduction systems for reversed phase LC-ICP-MS of selenium using large amounts of methanol—comparison of systems based on membrane desolvation, a spray chamber and direct injection

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Abstract

Three sample introduction systems were compared for their efficiency in introducing a variety of biologically relevant selenium species in organic solutions with the purpose of mimicking the effluent from a chromatographic system. The sample introduction systems consisted of a microconcentric nebuliser (MCN) in combination with a membrane desolvator (MD), a MCN in combination with a cyclonic spray chamber (CS) and a direct injection nebuliser (DIN), respectively. Thirteen selenium standards diluted in 50% methanol were introduced in each system at a flow rate of 50 μl min−1. Except for the selenite and methaneseleninic acid standards that were lost in the MCN-MD system, similar normalized response factors were achieved for the standards regardless of the sample introduction system. When trimethylselenonium and selenate standards were continuously infused in a linear methanol gradient from 10 to 90%, the signal was almost unaffected when the MCN-MD was used as interface. The signals from the MCN-CS introduction system were almost unaffected in methanol concentrations between 10 and 40%, after which they slowly declined. The signal from the DIN showed a plateau in signal intensity at methanol concentrations between 35 and 70%. Detection limits in flow injection analysis, expressed as the concentration that would result in a signal equivalent to three times the peak to peak noise of the baseline, were estimated to be 200, 150 and 100 ng Se L−1 for the MCN-CS, MCN-MD and DIN, respectively. The independence of the signal from the methanol concentration in the eluent allowed quantification of human selenoprotein P isoforms by gradient microbore reversed phase chromatography (μRPC) on the basis of an internal trimethylselenonium standard. The MCN-MD system was applied for μRPC analysis of 77Se enriched yeast samples and more than 30 selenium containing compounds were separated using a linear gradient from 5% to 95% methanol, some of the selenium species eluting in 85% methanol.

Graphical abstract: Sample introduction systems for reversed phase LC-ICP-MS of selenium using large amounts of methanol—comparison of systems based on membrane desolvation, a spray chamber and direct injection

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Publication details

The article was received on 11 Oct 2004, accepted on 28 Feb 2005 and first published on 14 Mar 2005


Article type: Paper
DOI: 10.1039/B415717F
Citation: J. Anal. At. Spectrom., 2005,20, 410-416
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    Sample introduction systems for reversed phase LC-ICP-MS of selenium using large amounts of methanol—comparison of systems based on membrane desolvation, a spray chamber and direct injection

    L. Bendahl and B. Gammelgaard, J. Anal. At. Spectrom., 2005, 20, 410
    DOI: 10.1039/B415717F

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