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Issue 21, 2005
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Reductive activation of nitrate reductases

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Abstract

Protein film voltammetry of Paracoccus pantotrophus respiratory nitrate reductase (NarGH) and Synechococcus elongatus assimilatory nitrate reductase (NarB) shows that reductive activation of these enzymes may be required before steady state catalysis is observed. For NarGH complementary spectroscopic studies suggest a structural context for the activation. Catalytic protein film voltammetry at a range of temperatures has allowed quantitation of the activation energies for nitrate reduction. For NarGH with an operating potential of ca. 0.05 V the activation energy of ca. 35 kJ mol−1 is over twice that measured for NarB whose operating potential is ca. −0.35 V.

Graphical abstract: Reductive activation of nitrate reductases

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Publication details

The article was received on 19 Apr 2005, accepted on 13 Jun 2005 and first published on 26 Sep 2005


Article type: Paper
DOI: 10.1039/B505530J
Citation: Dalton Trans., 2005,0, 3580-3586
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    Reductive activation of nitrate reductases

    S. J. Field, N. P. Thornton, L. J. Anderson, A. J. Gates, A. Reilly, B. J. N. Jepson, D. J. Richardson, S. J. George, M. R. Cheesman and J. N. Butt, Dalton Trans., 2005, 0, 3580
    DOI: 10.1039/B505530J

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