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Issue 21, 2005
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Dioxygen reduction by multi-copper oxidases; a structural perspective

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The multi-copper oxidases oxidise substrate molecules by accepting electrons at a mononuclear copper centre and transferring them to a trinuclear centre. Dioxygen binds to the trinuclear centre and, following the transfer of four electrons, is reduced to two molecules of water. The precise mechanism of this reduction has been unclear, but recent X-ray structural studies using the CotA endospore coat protein from Bacillus subtilis have given further insights into the principal stages. It is proposed that the mechanism involves binding of the dioxygen into the trinuclear centre so that it is sited approximately symmetrically between the two type 3 copper ions with one oxygen atom close to the type 2 copper ion. Further stages involve the formation of a peroxide intermediate and following the splitting of this intermediate, the migration of the hydroxide moieties towards the solvent exit channel. The migration steps are likely to involve a movement of the type 2 copper ion and its environment. Details of a putative mechanism are described herein based both on structures already reported in the literature and on structures of the CotA protein in the oxidised and reduced states and with the addition of peroxide and the inhibitor, azide.

Graphical abstract: Dioxygen reduction by multi-copper oxidases; a structural perspective

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Publication details

The article was received on 06 Apr 2005, accepted on 10 Jun 2005 and first published on 27 Sep 2005

Article type: Paper
DOI: 10.1039/B504806K
Citation: Dalton Trans., 2005, 3507-3513
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    Dioxygen reduction by multi-copper oxidases; a structural perspective

    I. Bento, L. O. Martins, G. Gato Lopes, M. Arménia Carrondo and P. F. Lindley, Dalton Trans., 2005, 3507
    DOI: 10.1039/B504806K

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