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Issue 19, 2005
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Conformation of glycosaminoglycans by ion mobility mass spectrometry and molecular modelling

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Abstract

We have performed conformational analyses of heparin-derived oligosaccharide ions in the gas phase using a combination of ion-mobility mass spectrometry and molecular modelling. Negative mode electrospray ionisation was used to generate singly (disaccharide, [C12H15NO19S3Na3]) and doubly charged (tetrasaccharides, [C24H30N2O38S6Na6]2− and [C24H31N2O35S5Na5]2−) ions containing three and six Na+ ions, respectively. Good agreement was obtained between the experimental and theoretical cross sections. The latter were obtained using modelled structures generated by the AMBER-based force field. Analysis of the conformations of the oligosaccharide ions shows that sodium cations play a major role in stabilizing these ions in the gas phase. This was seen in the formation of oligomers of the disaccharide ion and “compact” structures of tetrasaccharide ions. Interestingly, the gas phase conformations of the three tetrasaccharide ions with different primary structures were significantly different.

Graphical abstract: Conformation of glycosaminoglycans by ion mobility mass spectrometry and molecular modelling

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Publication details

The article was received on 17 Jun 2005, accepted on 04 Aug 2005 and first published on 22 Aug 2005


Article type: Paper
DOI: 10.1039/B508644B
Citation: Phys. Chem. Chem. Phys., 2005,7, 3464-3471
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    Conformation of glycosaminoglycans by ion mobility mass spectrometry and molecular modelling

    L. Jin, P. E. Barran, J. A. Deakin, M. Lyon and D. Uhrín, Phys. Chem. Chem. Phys., 2005, 7, 3464
    DOI: 10.1039/B508644B

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