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Issue 7, 2005
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Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

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Abstract

Infrared spectra of a 104 amino-acid protein in the gas phase as a function of its charge state are presented. The spectra contain clearly resolvable bands in the amide I and II spectral regions, as well as a band at 1483 cm−1, which is not observed in solution phase spectroscopy and is especially prominent for the higher charge states. Compared to solution, the amide I band is blue-shifted and the amide II band red-shifted, as expected for species in an environment with reduced hydrogen bonding. The band positions are suggestive of a mostly α-helical structure of the protein and their widths are comparable to those in solution, suggesting a similar conformational distribution.

Graphical abstract: Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

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Publication details

The article was received on 15 Feb 2005, accepted on 03 Mar 2005 and first published on 07 Mar 2005


Article type: Communication
DOI: 10.1039/B502322J
Citation: Phys. Chem. Chem. Phys., 2005,7, 1345-1348
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    Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

    J. Oomens, N. Polfer, D. T. Moore, L. van der Meer, A. G. Marshall, J. R. Eyler, G. Meijer and G. von Helden, Phys. Chem. Chem. Phys., 2005, 7, 1345
    DOI: 10.1039/B502322J

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