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Issue 8, 2005
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Evaluation of an accurate calibration and spectral standardization procedure for Raman spectroscopy

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Abstract

Due to modern developments Raman spectroscopy has evolved into a fast vibrational technique. Detailed fingerprints in combination with non-destructivity and minimal sample preparation has allowed the construction of reference libraries in a variety of research fields. Long-term stability and comparability are important characteristics when developing reference libraries. In addition, small shifts in highly similar spectra of different samples may limit the full potential of Raman spectroscopy. Since libraries often contain a large number of different and/or highly similar spectra, it is important that each data point in all the spectra corresponds to the exact Raman wavenumber. This is often not the case, due to shifts in optical pathway and/or shifts in laser wavelength. This paper describes a complete calibration protocol (wavelength and intensity) and evaluates the procedure for both short and long term stability, by means of 60 randomly selected measurement sessions spread over a period of nine months. A two-step standardization procedure is proposed to deal with spectral shifts.

Graphical abstract: Evaluation of an accurate calibration and spectral standardization procedure for Raman spectroscopy

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Publication details

The article was received on 10 Mar 2005, accepted on 02 Jun 2005 and first published on 04 Jul 2005


Article type: Paper
DOI: 10.1039/B503624K
Citation: Analyst, 2005,130, 1204-1214
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    Evaluation of an accurate calibration and spectral standardization procedure for Raman spectroscopy

    D. Hutsebaut, P. Vandenabeele and L. Moens, Analyst, 2005, 130, 1204
    DOI: 10.1039/B503624K

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