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Issue 7, 2005
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Near infrared Raman spectroscopic mapping of native brain tissue and intracranial tumors

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Abstract

This study assessed the diagnostic potential of Raman spectroscopic mapping by evaluating its ability to distinguish between normal brain tissue and the human intracranial tumors gliomas and meningeomas. Seven Raman maps of native specimens were collected ex vivo by a Raman spectrometer with 785 nm excitation coupled to a microscope with a motorized stage. Variations within each Raman map were analyzed by cluster analysis. The dependence of tissue composition on the tissue type in cluster averaged Raman spectra was shown by linear combinations of reference spectra. Normal brain tissue was found to contain higher levels of lipids, intracranial tumors have more hemoglobin and lower lipid to protein ratios, meningeomas contain more collagen with maximum collagen content in normal meninges. One sample was studied without freezing. Whereas tumor regions did not change significantly, spectral changes were observed in the hemoglobin component after snap freezing and thawing to room temperature. The results constitute a basis for subsequent Raman studies to develop classification models for diagnosis of brain tissue.

Graphical abstract: Near infrared Raman spectroscopic mapping of native brain tissue and intracranial tumors

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Publication details

The article was received on 05 Jan 2005, accepted on 03 May 2005 and first published on 24 May 2005


Article type: Paper
DOI: 10.1039/B419232J
Citation: Analyst, 2005,130, 1070-1077
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    Near infrared Raman spectroscopic mapping of native brain tissue and intracranial tumors

    C. Krafft, S. B. Sobottka, G. Schackert and R. Salzer, Analyst, 2005, 130, 1070
    DOI: 10.1039/B419232J

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