Molecular imprinting-chemiluminescence determination of trimethoprim using trimethoprim-imprinted polymer as recognition material

Abstract

A new molecular imprinting-chemiluminescence method for the determination of trimethoprim was developed, in which trimethoprim-imprinted polymer was used as the molecular recognition material and the CL reaction of trimethoprim with potassium permanganate in acidic medium was used as the detection system. The CL intensity responds linearly to the concentration of trimethoprim within the 5.0 × 10⁻⁸–5.0 × 10⁻⁶ g mL⁻¹ range (r = 0.9983) with a detection limit of 2 × 10⁻⁸ g mL⁻¹. The relative standard deviation for the determination of 1.0 × 10⁻⁷ g mL⁻¹ trimethoprim solutions is 4.8% (n = 9). The method has been applied to the determination of trimethoprim in pharmaceutical preparations and body fluids, and satisfactory results were obtained.