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Issue 8, 2004
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Time-domain fluorescence lifetime imaging applied to biological tissue

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Abstract

Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue.

Graphical abstract: Time-domain fluorescence lifetime imaging applied to biological tissue

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Publication details

The article was received on 18 Dec 2003, accepted on 10 May 2004 and first published on 04 Jun 2004


Article type: Perspective
DOI: 10.1039/B316456J
Citation: Photochem. Photobiol. Sci., 2004,3, 795-801
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    Time-domain fluorescence lifetime imaging applied to biological tissue

    D. Elson, J. Requejo-Isidro, I. Munro, F. Reavell, J. Siegel, K. Suhling, P. Tadrous, R. Benninger, P. Lanigan, J. McGinty, C. Talbot, B. Treanor, S. Webb, A. Sandison, A. Wallace, D. Davis, J. Lever, M. Neil, D. Phillips, G. Stamp and P. French, Photochem. Photobiol. Sci., 2004, 3, 795
    DOI: 10.1039/B316456J

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