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Issue 4, 2004
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Micro patterning of active proteins with perforated PDMS sheets (PDMS sieve)

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Abstract

We propose a novel technique for patterning active proteins on a glass substrate using a perforated polydimethylsiloxane (PDMS) sheet–sieve. The sieve, which has tapering holes, is fabricated by spin-coating PDMS on a pyramidal-shaped mold. By means of this sieve, FITC (fluorescent isothiocyanate, bovine)–albumin was successfully spotted in a 5 × 5 µm2 area in an array. The patterned spots were perfectly isolated, which eliminates the problem of non-specific binding of proteins to undesired areas. To show that proteins maintained their activity after the patterning, we used F1-ATPase biomolecular motors; their activity can easily be verified by observing their rotary motion after patterning. Selective patterning with three kinds of fluorescent micro beads indicated the possibility of patterning of different proteins on the same substrate by using the sieve.

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Publication details

The article was received on 16 Jan 2004, accepted on 16 Mar 2004 and first published on 06 Apr 2004


Article type: Paper
DOI: 10.1039/B400774C
Citation: Lab Chip, 2004,4, 333-336
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    Micro patterning of active proteins with perforated PDMS sheets (PDMS sieve)

    K. Atsuta, H. Noji and S. Takeuchi, Lab Chip, 2004, 4, 333
    DOI: 10.1039/B400774C

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