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Issue 14, 2004
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Transfection of cells with custom-made calcium phosphate nanoparticles coated with DNA

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Abstract

Functionalised nanoparticles of calcium phosphate were prepared by controlled precipitation from aqueous solution, followed by coating with DNA. A successful transfection of transformed human endothelial cells was accomplished by adding the dispersion of nanoparticles to the cell culture. The functionalised nanoparticles formed a stable colloid and did not lose their ability for cell transfection during 2–3 weeks of storage. The nanoparticles were approximately spherical with diameters of 10 to 20 nm and covered and stabilised by a hull of DNA.

Graphical abstract: Transfection of cells with custom-made calcium phosphate nanoparticles coated with DNA

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Publication details

The article was received on 03 Feb 2004, accepted on 17 Mar 2004 and first published on 23 Apr 2004


Article type: Paper
DOI: 10.1039/B401644K
Citation: J. Mater. Chem., 2004,14, 2213-2217
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    Transfection of cells with custom-made calcium phosphate nanoparticles coated with DNA

    T. Welzel, I. Radtke, W. Meyer-Zaika, R. Heumann and M. Epple, J. Mater. Chem., 2004, 14, 2213
    DOI: 10.1039/B401644K

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